Search found 65 matches

by SororSaudade
Fri Feb 02, 2007 11:52 am
Forum: Molecular Biology
Topic: How to find the con of DNA if extractd sample is too less??
Replies: 8
Views: 3652

It would definitely be better to use a kit since you would get higher amounts of DNA , thought I don't know which is the most apropriate to isolate DNA from blood. But, if it depends on the amount of parasites, I think using a kit won't solve the variation problem. By quantifying in agarose gel you ...
by SororSaudade
Thu Feb 01, 2007 6:08 pm
Forum: Botany Discussion
Topic: how r weedicides weed specific??
Replies: 9
Views: 6228

well, I can't understand your point either :P

I've never heard of auxins as "weedicides", but what I ment is that smaller plants could be less tolerant to certain amounts of auxins, but yet this may be a stupid answer, I don't know.

If you find the right answer please let me know :)
by SororSaudade
Thu Feb 01, 2007 11:45 am
Forum: Botany Discussion
Topic: how r weedicides weed specific??
Replies: 9
Views: 6228

maybe, as weeds are smaller, they are easily afected by smaller amounts of those auxins (though i'm not sure).
by SororSaudade
Thu Feb 01, 2007 11:30 am
Forum: Molecular Biology
Topic: How to find the con of DNA if extractd sample is too less??
Replies: 8
Views: 3652

you can see the concentration of your sample in an agarose gel by comparing it with, for example, lambda of known concentrations, or a specific ladder.

How do you extract your DNA? What kind of DNA are you talking about?
by SororSaudade
Thu Feb 01, 2007 12:47 am
Forum: Botany Discussion
Topic: polar transport of auxin
Replies: 9
Views: 10080

by SororSaudade
Thu Feb 01, 2007 12:41 am
Forum: Botany Discussion
Topic: polar transport of auxin
Replies: 9
Views: 10080

doesn't it have to do with changes between basic and acidic forms of auxins? I can't remember that well what I learned in college but I think it had something to do with this, which allowed a continuous flow of auxins (since there was always a gradient of the two forms). Forgive me if I'm wrong or i...
by SororSaudade
Wed Jan 31, 2007 9:02 pm
Forum: Molecular Biology
Topic: Restriction Mapping
Replies: 6
Views: 2995

Agronomy, I believe you can easily find a restriction map of that plasmid on the web, so that you can answer your question :P
by SororSaudade
Fri Jan 26, 2007 1:37 pm
Forum: Botany Discussion
Topic: The Photosynthesis Process
Replies: 5
Views: 5596

z-scheme is just for the first part of photosynthesis.
I believe you can easily google it... there must be a lot of information about this.
by SororSaudade
Mon Jan 22, 2007 5:17 pm
Forum: Botany Discussion
Topic: algae
Replies: 14
Views: 14964

sunnygirl wrote:So we knw that both red and green algea obtain their food by photosynthesis, so why can rd algea survive in deep water and green cannot?


they have diferent pigments that capture light at diferent wave lengths.
by SororSaudade
Mon Jan 22, 2007 4:42 pm
Forum: Molecular Biology
Topic: Need advice in primer designing
Replies: 3
Views: 2189

have you tried different annealing temperatures?

hopefully this paper will help you as it did to me:

http://bioinformatics.oxfordjournals.or ... t/bti633v1
by SororSaudade
Thu Jan 18, 2007 12:18 am
Forum: Molecular Biology
Topic: agarose gel
Replies: 7
Views: 7069

i've always heard that EtBr is heat-labile (its altered by heat).
i also think that it doesn't really matter if you use TBE or TAE... at least for DNA electrophoresis.
by SororSaudade
Wed Jan 17, 2007 4:17 pm
Forum: Molecular Biology
Topic: loading buffer
Replies: 2
Views: 1991

http://www.thelabrat.com/protocols/XCBBDNAload.shtml

I do it without SDS for DNA electrophoresis.