Search found 65 matches

by SororSaudade
Tue Feb 27, 2007 5:50 pm
Forum: Molecular Biology
Topic: PCR with only one primer
Replies: 17
Views: 30763

olha... tinónis pra ti!!!!!!!!! :lol:

nha nha nha nha nha
by SororSaudade
Sat Feb 24, 2007 1:56 pm
Forum: Molecular Biology
Topic: smear after restriction digestion
Replies: 23
Views: 33157

does the restriction enzyme you're using have star activity? It could be cutting where it shouldn't.
by SororSaudade
Thu Feb 22, 2007 10:29 am
Forum: Botany Discussion
Topic: Seaweeds!
Replies: 14
Views: 11022

can you show a picture of those seaweeds?
by SororSaudade
Thu Feb 22, 2007 10:28 am
Forum: Molecular Biology
Topic: smear after restriction digestion
Replies: 23
Views: 33157

probably DNA degradation
by SororSaudade
Thu Feb 22, 2007 10:26 am
Forum: Molecular Biology
Topic: plasmid prcipitation
Replies: 6
Views: 4528

You should also add sodium acetate (1/10 of the final volume). I think this is just to stabilize the plasmid and not to improve percipitation, though I'm not quite sure.

I generally use for transfection plasmids dissolved in TE... is there any advantage in using PBS?
by SororSaudade
Mon Feb 12, 2007 3:04 pm
Forum: Molecular Biology
Topic: ratios for ligation reactions
Replies: 2
Views: 2270

well, i'm working with a 3,7kb vector and a 1,6kb insert and 1:1 and 1:3 don't seem to work. I've now tried 1:5 and this seems to work but wih very low efficiency. I'm trying to ligate blunt ends... maybe that's why the ratios have to be higher.. It would be very helpful if it was possible to calcul...
by SororSaudade
Mon Feb 12, 2007 1:10 pm
Forum: Molecular Biology
Topic: ratios for ligation reactions
Replies: 2
Views: 2270

ratios for ligation reactions

Hello!

I was wondering if it is possible to know the best vector:insert ratio to use in a ligation reaction, knowing the sizes of both fragments.
Is there any way to calculate it, or you just have to try different ratios until you get the right one?

Thanks :)
by SororSaudade
Fri Feb 09, 2007 1:17 pm
Forum: Botany Discussion
Topic: Need Local (urdu names) of trees
Replies: 3
Views: 8164

what's an urdu name? (sorry for my ignorance :P )
by SororSaudade
Wed Feb 07, 2007 11:59 pm
Forum: Molecular Biology
Topic: PCR with only one primer
Replies: 17
Views: 30763

unfortunately the size is exactly the same and the sequence only differs in about 10 nucleotides (in a 1,8kb fragment) and there won't be new restriction sites. well... I think I just found another way to do what I want, by linearly amplify one strand and purify it... then, after the PCR, I will onl...
by SororSaudade
Wed Feb 07, 2007 2:10 pm
Forum: Botany Discussion
Topic: Microwaved Water
Replies: 26
Views: 22408

I know you didn't do it. I was just asking if you knew something more about it.
I totally agree with you about the controls... it could be very interesting though.
by SororSaudade
Tue Feb 06, 2007 10:03 am
Forum: Botany Discussion
Topic: Microwaved Water
Replies: 26
Views: 22408

it seems to me you can´t take any conclusions based on only two plants... it may have nothing to do with the water.
Are there any more details available?
by SororSaudade
Sun Feb 04, 2007 9:10 pm
Forum: Botany Discussion
Topic: how r weedicides weed specific??
Replies: 9
Views: 6228

nice to know that :)

Thanks for sharing the right answer!