Search found 14 matches

by molecular newbie
Tue Jun 27, 2006 11:21 pm
Forum: Molecular Biology
Topic: Using primers after DNA modification
Replies: 6
Views: 2985

they are 3 different primers that cleave 3 different genes from the DNA strand. They dont differ much , and they have enough complentary base pairs to cleave that specific gene. They are commercial primers and therfore they are already mixed togther , i just add them before i add my master mix. One ...
by molecular newbie
Tue Jun 27, 2006 7:17 pm
Forum: Molecular Biology
Topic: Using primers after DNA modification
Replies: 6
Views: 2985

Using primers after DNA modification

Im relatively new to this stuff but ive been doing research for the past month , working with 3 commerical primers and control/experimental DNA samples. I use the methylated specific PCR procedure and then gel electrophorisis for result. MY PROBLEM is that im not get consistent bands and even someti...