Search found 45 matches

by bravebeaker
Mon Mar 04, 2013 8:02 am
Forum: Molecular Biology
Topic: significant difference
Replies: 2
Views: 2767

significant difference

Hi everyone, Sorry if this is covered before but I was thinking about which statistical test to use to check the significance in the experiment. I read some papers for what they are using and found out that some publications are not using any test except mentioning the SE. I have two groups of plant...
by bravebeaker
Wed Jan 30, 2013 4:27 am
Forum: Molecular Biology
Topic: RT-PCR
Replies: 9
Views: 8698

yes
by bravebeaker
Tue Jan 29, 2013 1:19 am
Forum: Molecular Biology
Topic: RT-PCR
Replies: 9
Views: 8698

Now I see your point, but in my case I use the primer mix that comes with the RT kit by Takara..
by bravebeaker
Mon Jan 28, 2013 10:18 am
Forum: Molecular Biology
Topic: RT-PCR
Replies: 9
Views: 8698

I am saying based on the kit I am using which is SYBR green by TOYOBO and also many qPCR protocols over the internet as well as papers indicate the use of a short amplicon between 80 to 200 bp... what do you think? maybe banuy's experiment is different?
by bravebeaker
Wed Jan 23, 2013 8:24 am
Forum: Molecular Biology
Topic: RT-PCR
Replies: 9
Views: 8698

as far as i know and correct me if i am wrong, RT-PCR amplification products should be short, not too short but not too long, if I understood correctly you are amplifying a 2Kb amplicon I think that is very very long.
by bravebeaker
Wed Jan 23, 2013 8:20 am
Forum: Molecular Biology
Topic: Does my protein bind DNA?
Replies: 12
Views: 14913

would you kindly inform us about your result I am interested of what will you get since you dont know the DNA sequence, I have a research case where I know the sequence but dont know the protein..
by bravebeaker
Wed Jan 09, 2013 7:22 am
Forum: Molecular Biology
Topic: The same old silly question about molarities
Replies: 3
Views: 3520

Re: The same old silly question about molarities

Well Jack bean... I was waiting with anticipation for your reply actually but not kind of that reply.. very elaborative :P but thanks anyways :D
by bravebeaker
Wed Dec 26, 2012 4:47 am
Forum: Molecular Biology
Topic: qPCR
Replies: 2
Views: 3839

1. extracted RNA --> DNase treatment --> RT to synthesize cDNAs
2. use same primers to amplify your target by PCR
3. extract bands from gel and purify target DNA
4. use the purified DNA for making a standard curve.
5. runa a qPCR
by bravebeaker
Wed Dec 26, 2012 3:35 am
Forum: Molecular Biology
Topic: The same old silly question about molarities
Replies: 3
Views: 3520

The same old silly question about molarities

Hi all, I have a question and I would like you to help me figure it out. Sometimes we read about solution contents for example the enzyme solution used in protoplast isolation, well in the paper its written like this 20 mM MES 0.4 M mannitol 20 mM KCl 10 mM CaCl2 0.1% BSA 1.5% Cellulase and so on......
by bravebeaker
Sat Dec 01, 2012 7:13 am
Forum: Molecular Biology
Topic: Molecular bio questions
Replies: 2
Views: 2766

i think... you should google them :)
by bravebeaker
Sat Dec 01, 2012 7:00 am
Forum: Molecular Biology
Topic: Cloning/Transformation question
Replies: 4
Views: 4512

also sometimes when i ligate short oligos, i use different inster:vector ratios into different plates just to see the pattern.. hope it helps too!
by bravebeaker
Thu Oct 25, 2012 6:46 am
Forum: General Discussion
Topic: Is there an insect that behaves this way?
Replies: 6
Views: 7175

two options come to my mind
1. sick tinkerbell.
or
2. mini green latern