Search found 90 matches

by oppox
Wed May 31, 2006 5:26 pm
Forum: Molecular Biology
Topic: Vector is 80X larger than insert, what molar ratio to use?
Replies: 9
Views: 9288

The thing that manuals warn you about is not using too much insert if its chloroform extracted and then its the volume of PCR product that could mess it up. Ive seen manuals suggest like a molar ratio of 10:1. But since your insert is so small the volume gets very small, im taking 1 microliter inser...
by oppox
Wed May 31, 2006 5:16 pm
Forum: Molecular Biology
Topic: What is an initiation sequence?
Replies: 1
Views: 1945

yea its a short sequence in the DNA that RNA polymerase recoqnizes,
its called a promotor site, for an example the TATA box (TATAA).
by oppox
Fri May 26, 2006 10:48 am
Forum: Molecular Biology
Topic: a couple of questions about cloning.
Replies: 2
Views: 1972

a couple of questions about cloning.

Im having troubles with a gene, an AT rich gene. problem 1. I have cloned my gene (700 bp) into a petblue vector (novagen), an open vector with a T overhang making it possible to ligate it with any fragments amplified with taq poly. I have the correct insert according to sequencing, but I cant expre...
by oppox
Sat May 13, 2006 12:03 pm
Forum: Molecular Biology
Topic: AT-Rich genes (again)
Replies: 1
Views: 1494

AT-Rich genes (again)

Hello im new here, so you have to ecuse me if u have heard my question before but I couldnt find any realated topics except one. Anyway to the question, Im writing my final thesis and my prof wanted me to bring up the problems with AT rich genes. Thing is I cant find any reports or articles on the s...
by oppox
Sat May 13, 2006 11:37 am
Forum: Molecular Biology
Topic: AT rich DNA
Replies: 3
Views: 2537

do u got the insert in a separate vector? are u going to cut it out, then ligate it into the other vector? Or are u trying to pick it up with PCR? I have found a couple of problems with PCR on AT rich regions, dunno if they are real problems though , I mean I dont know if I had a problem with it, bu...