Search found 90 matches

by oppox
Thu Sep 14, 2006 2:58 pm
Forum: Bioinformatics
Topic: DNA analysis software
Replies: 3
Views: 3792

I know a homepage that has alot of that kind of things, similarities between two nucleotide sequences and so on. http://www.expasy.org
by oppox
Mon Sep 11, 2006 2:09 pm
Forum: Molecular Biology
Topic: Memorising 20 Amino Acids!
Replies: 11
Views: 30781

I agree with lilkim, make cards with them and just study study study. I had some remembering rules for some of them. Like proline, sounds like brolin a swedish soccer player, he plays football and well proline looks like a football and so on. study them and try to make up your own remembering things...
by oppox
Mon Sep 11, 2006 11:12 am
Forum: Molecular Biology
Topic: # of protons, electrons, and neutrons
Replies: 5
Views: 8089

well its pretty easy, the atomic number tells u how many protons, if the atom is neutral it has the same number of electrons. The charge is regulated by the electrons, if it is a singel negative charge it has one electron more and so on. substract the atom number from the atom weight to find out how...
by oppox
Sat Sep 09, 2006 8:49 pm
Forum: Bioinformatics
Topic: Good software!
Replies: 2
Views: 3361

There are programs that allows u to superimpose one sequence in fasta onto a known structure, there are also a homepage that predict the structure. Though u never get the result as a picture. I dont remember their names cause I have it on my comp at school, I can get the name tomorrow. However I thi...
by oppox
Thu Sep 07, 2006 8:33 am
Forum: Molecular Biology
Topic: Ap Bio Help!
Replies: 5
Views: 6661

well nothing of what u write is wrong. Water has one of the highest (the highest?) specific heat capacities. How much work are u going to spend writing this essay, are u going to explain what dipolar means and how it forms and why it has high heat capacity and so on? I think u have more then enough ...
by oppox
Mon Sep 04, 2006 8:22 am
Forum: Molecular Biology
Topic: Two problems im trying to figure out.
Replies: 0
Views: 913

Two problems im trying to figure out.

Hello Im having a little problem. I trying to express a protein from a gete in a pET28 vector. Im growing it in Bl21DE3 and inducing it with IPTG. I get no protein when I analyze it on a SDS-gel, any suggestions? Also, Im having some problems preparing the samples for the SDS gel, that is when Im st...
by oppox
Fri Sep 01, 2006 2:55 pm
Forum: Molecular Biology
Topic: Taq activity?
Replies: 4
Views: 2277

on the other hand after some thoughts, in a ordinary PCR cycle the annealing temp is about 50 deg and then rised to 72 for taq to begin. So I dont know but it might be a problem there. If taq was active at so much lower temps, it would already have replicated the whole gene before the temp hit 72, a...
by oppox
Thu Aug 31, 2006 11:55 pm
Forum: Molecular Biology
Topic: Taq activity?
Replies: 4
Views: 2277

Why are u planning on going so low?
I cant see why you shouldnt try it out, maybe u should increase your extansion time though, to 5-6 min perhaps.
by oppox
Tue Aug 29, 2006 8:24 am
Forum: Molecular Biology
Topic: Name of gene amplified in PCR
Replies: 2
Views: 1546

Do u mean template or? I dont get the question :)
by oppox
Wed Aug 23, 2006 11:07 pm
Forum: Molecular Biology
Topic: synthetic enzyme substrates
Replies: 9
Views: 3786

yea I think he knew :wink: . I think the best u can do is search it, search googles and in journals etc. I dont think u will find an overview in one place since its alot of substrates for alot of different proteins.
by oppox
Sat Aug 19, 2006 3:42 pm
Forum: Molecular Biology
Topic: PCR problems. I need to know what is going on.....
Replies: 9
Views: 6519

hmm yea I re-read my answer and yea purifying it didnt make any sence. I picked up a gene from a genomic DNA were the primers needed had a annealing temp of 63 deg, I used two pairs of primers, the first ones had extra GC in the ends to raise the annealing temp and in the next run I used the primers...
by oppox
Thu Aug 17, 2006 11:41 pm
Forum: Molecular Biology
Topic: PCR problems. I need to know what is going on.....
Replies: 9
Views: 6519

I cant answer to the preparation steps before the PCR, one thing u can try though which is pretty fast. Do an extra heating to 95 deg after the PCR program and then cool it down. You have a pretty low working temp though and maybe it causes some problem. what polymerase do u use? Do u have both wilt...