Search found 90 matches

by oppox
Tue Oct 10, 2006 10:22 pm
Forum: Molecular Biology
Topic: Twisted double Helix
Replies: 7
Views: 5828

evolution, DNA is more stable that way then RNA. maybe its a more energetic conformation, the H-bonds between the bases forces it to adopt that structure. RNA is "always" single stranded so it doesnt have the pairing bases. Thats what I could come up with at the moment :)
by oppox
Tue Oct 10, 2006 9:03 pm
Forum: Botany Discussion
Topic: Photosynthesis
Replies: 9
Views: 13486

ok then I understand the question, on the other hand its not green its absorbing, its absorbing al colors except green.
by oppox
Tue Oct 10, 2006 8:04 am
Forum: Botany Discussion
Topic: Photosynthesis
Replies: 9
Views: 13486

all colors absorb light (with white as an exception). I dont understand the question, but that may be because Im not the best in english. But its not so hard to look it up, here is some keywords: wavelength colors spectra
by oppox
Mon Oct 09, 2006 6:55 am
Forum: Molecular Biology
Topic: Enzyme kinetics
Replies: 15
Views: 21880

then u know that in the future u can trust the L-B plot and it is more simple to use then the average thing u did :wink:
by oppox
Fri Oct 06, 2006 9:58 am
Forum: Molecular Biology
Topic: Enzyme kinetics
Replies: 15
Views: 21880

did you solve it? just make two columns, one with 1/[S] and one with 1/V and then just plot those values (reverse it so u get a positive line).
by oppox
Thu Oct 05, 2006 9:56 am
Forum: Molecular Biology
Topic: Enzyme kinetics
Replies: 15
Views: 21880

enzymemaster? no no get your ruler out and draw oldschool :)
by oppox
Wed Oct 04, 2006 3:25 pm
Forum: Molecular Biology
Topic: Enzyme kinetics
Replies: 15
Views: 21880

you cant say that Vmax is 40.5 because you dont know what happens when [S] gets larger. You have to plot it (L-burke plot), u cant solve it with the menten equation because Vmax is approached asymptotically and u dont get a difinitive value. if u plot it u get a straight line, if it obey michaelis-m...
by oppox
Sun Oct 01, 2006 1:16 am
Forum: General Discussion
Topic: I need your help guys!! Tell me how I did on my Bio test->
Replies: 13
Views: 20934

I would answer 8 with a C
by oppox
Fri Sep 29, 2006 11:59 pm
Forum: Molecular Biology
Topic: Methods for diet analysis
Replies: 3
Views: 2398

isnt ELISA just a spectrophotometer that can read elisa plates and on that plate u can bind things to antibodies. I dont know much of the other stuff though
by oppox
Wed Sep 27, 2006 5:37 pm
Forum: Molecular Biology
Topic: clonning
Replies: 2
Views: 1697

1. well I guess the enzymes could cut again if u dont, I dont remember how I did. Some enzymes can be stopped by for an example heating. you do best to download the manual to your enzymes
by oppox
Wed Sep 27, 2006 11:54 am
Forum: Molecular Biology
Topic: Refolding a protein
Replies: 0
Views: 900

Refolding a protein

Hello, I have a protein that has to be denaturated before purification. I have tried to refold it by quick dilution but then it aggregates again. My question is, should I try and refold it on the purification colonn (Ni, his-tag) instead (by slow dilution), is it a better way?
by oppox
Fri Sep 22, 2006 8:42 am
Forum: Molecular Biology
Topic: Help: Is species identification _at_ IR range useful?
Replies: 4
Views: 2860

hmmm I dont know anything about IR and I dont know if anyone in my corridor use it, we use CD to get a picture of the structure. But when I used to work in the lab at a dairy we used IR to determine how much fat the milk and so on contained.