Search found 90 matches

by oppox
Thu Oct 26, 2006 8:59 am
Forum: Molecular Biology
Topic: Using PCR to determine orientation of plasmid insert
Replies: 3
Views: 10418

Where the insert is located depends on where u cut the vector, Im not familiar with a way to locate it if u dont know where its located. But u can find out if it is your insert and if its turned in the right direction by using a primer that fits the vector closly to where u think the gene is and a p...
by oppox
Thu Oct 26, 2006 8:54 am
Forum: Molecular Biology
Topic: which amino acid cannot form helical structure
Replies: 4
Views: 2579

look at the structure of proline, it "forces" the chain into a bend that would break helical structure. Thus it can be found in turns/hairpins between helicals etc. Think of it like a u-turn :)
by oppox
Thu Oct 19, 2006 11:19 pm
Forum: Molecular Biology
Topic: here is an easy one for you
Replies: 2
Views: 1477

Then I was thinking in the right direction, thank you for making it more clear.
by oppox
Thu Oct 19, 2006 10:07 am
Forum: Molecular Biology
Topic: here is an easy one for you
Replies: 2
Views: 1477

here is an easy one for you

Im trying to fully understand this. I got a table with different enzymes and inhibitors. ........x........ y...........z. 1.....0,67.....42........0,17 2.....0,53.....13........0,29 where 1 and 2 are different inhibitors and x, y, z are different enzymes. the figures are Ki (microM). Its been some t...
by oppox
Wed Oct 18, 2006 11:50 pm
Forum: General Discussion
Topic: About PRIONS
Replies: 1
Views: 1282

like the mad cow disease
by oppox
Wed Oct 18, 2006 7:18 pm
Forum: Molecular Biology
Topic: cDNA integration into Plasmid
Replies: 3
Views: 1770

Ive done a lab where we created a cDNA library I think but it was a long time ago and I dont remember the details so here is a link to something similar.

http://courses.washington.edu/dmandoli/ ... ibrary.pdf
by oppox
Wed Oct 18, 2006 3:38 pm
Forum: Molecular Biology
Topic: pcr
Replies: 1
Views: 1601

use primers that fits the gene
by oppox
Mon Oct 16, 2006 10:53 pm
Forum: Molecular Biology
Topic: Can some one help me with these Molecular Biology questions?
Replies: 4
Views: 1936

1. yes its 3 and its called a codon also search the word reading frame. 2. antisense strand is the other strand in a double DNA strand. 3. see 1, search for codon table, for an example look in wikipedia. 4. search for BamH1 and HindIII at w these questions arent hard to find the answers to if u look...
by oppox
Mon Oct 16, 2006 9:00 am
Forum: Molecular Biology
Topic: Primer selection question (homework)
Replies: 1
Views: 1183

They made it simplier by writing the start of the gene in bold letters. The extra G in the end is for raising the annealing temp in the PCR and is not found in the gene. The gene and vector is written 5'-3' as the primers. A gene is inserted 5'-3' into a vector, that is as its written. The forward p...
by oppox
Thu Oct 12, 2006 8:50 pm
Forum: Molecular Biology
Topic: Draw plasmid..free softwares
Replies: 2
Views: 4700

draw plasmid, how do u mean?
by oppox
Thu Oct 12, 2006 7:18 pm
Forum: Molecular Biology
Topic: Problem with Ligation and Transfection
Replies: 1
Views: 1474

I have had problem with pET28 too, restriction digestion. You can check your cut material on an agarose gel for more bands then one. I left the restriction digestion and used a pET-blue vector, I got the insert in the right place but I couldnt express the gene. So my second coice was t try a restric...
by oppox
Tue Oct 10, 2006 11:27 pm
Forum: Molecular Biology
Topic: Beta pleated sheet vs alpha helix
Replies: 5
Views: 8190

thats a hard question and its not possible to answer diffenatly. Some aminoacids are said to be for an example helical inducers, like leucine and alanine. proline for an example is often found in hairpins. But there is no rule without exceptions so...