Search found 5720 matches

by MrMistery
Thu Feb 04, 2010 10:38 pm
Forum: Cell Biology
Topic: synchronising cell cultures
Replies: 2
Views: 2076

we used to starve them of serum to synchronize them. can't you just do that?
by MrMistery
Thu Feb 04, 2010 10:37 pm
Forum: Cell Biology
Topic: Vacuoles in Plants and Animals
Replies: 12
Views: 14654

@jwalin that's just a wrong way of saying yet. Of course I can't say it's wrong because you can just change the definition of a vacuole and it's fine. But nowadays in cell biology we are done with identifying things by the way they look. We identify things based on their origins and the proteins the...
by MrMistery
Wed Feb 03, 2010 1:21 pm
Forum: Cell Biology
Topic: Vacuoles in Plants and Animals
Replies: 12
Views: 14654

excuse me? What might "recognize a sentence" mean?
by MrMistery
Tue Feb 02, 2010 8:24 pm
Forum: Cell Biology
Topic: Vacuoles in Plants and Animals
Replies: 12
Views: 14654

it is important. you can call it whatever you want, but people seem to make a big confusion between a vacuole and a vesicle. i would have nothing against calling them lysosomes in all cells or calling them vacuoles in all cells. The reason they are called different is of course historic - dating bac...
by MrMistery
Tue Feb 02, 2010 1:26 am
Forum: Botany Discussion
Topic: Plant Systematics
Replies: 2
Views: 3161

the part of "magnoliophyta" that you have to notice is not the magnolia part, it is the "phyta" part - that is what tells you what level in the classification you are at. And from what I know the latest classification divides them into amborella, water lilies, magnolias and eudic...
by MrMistery
Tue Feb 02, 2010 1:12 am
Forum: General Discussion
Topic: Are there any genes that are unique to yeast?
Replies: 8
Views: 3660

a lot of things are different between yeast and human. for example, being an ER person, i would look for the retention sequence at the end of ER-resident proteins. The ones in yeast cells have an HDEL at the end and the ones in human cells have a KDEL at the end.
by MrMistery
Tue Feb 02, 2010 1:04 am
Forum: Molecular Biology
Topic: Running SDS PAGE for the first time!
Replies: 9
Views: 8354

50 ng should be plenty if you have purified protein, though it also depends - coumassie in my experience can "go bad", especially since most labs reuse that stuff for a million years. But if that's not the case, you should be fine
by MrMistery
Tue Feb 02, 2010 12:56 am
Forum: Molecular Biology
Topic: Biology Lab Help - Protein Assays
Replies: 4
Views: 9292

do you know how a bradford assay works?
by MrMistery
Tue Feb 02, 2010 12:56 am
Forum: Cell Biology
Topic: GENERAL BIOLOGY
Replies: 12
Views: 8174

guys, is this really important? Who the hell cares what you call a macromolecule and what you don't call a macromolecule?
by MrMistery
Tue Feb 02, 2010 12:46 am
Forum: Cell Biology
Topic: Vacuoles in Plants and Animals
Replies: 12
Views: 14654

i think I may have said this probably at least 100 times on this forum, but I'll say it again: animal cells have no vacuoles. The vacuole in plant, fungi and protozoan cells is nothing but a modified lysosome. In animal cells they are called lysosomes, are generally a lot smaller and have different ...
by MrMistery
Sat Jan 30, 2010 4:27 pm
Forum: Cell Biology
Topic: cellular component
Replies: 3
Views: 2369

actually... Vesicles are little "transport and temporary storage" devices that the cell uses to transport stuff between the ER and the Golgi and the lysosomes, and only to take stuff to the plasma membrane. A vesicle is defined by its small size and by how it forms (through the formation o...
by MrMistery
Sun Jan 17, 2010 1:01 pm
Forum: Human Biology
Topic: Race and sound of voice.
Replies: 6
Views: 12698

nobody can deny that there genetic differences between the so-called "races" owing to their different genetic ancestries, but it is a fact that the largest percentage of genetic variation (over 70% if I remember correctly) is inside populations. Compared to that, differences between "...