Search found 14 matches

by dhkwak
Mon Feb 01, 2010 9:26 pm
Forum: Molecular Biology
Topic: Running SDS PAGE for the first time!
Replies: 9
Views: 8172

Re: Running SDS PAGE for the first time!

Thanks JackBean, I read somewhere that when the running solution contains Tris-Tricine, separate electrode buffers need to be implemented; conversely, when Tris-Glycine is used, the buffer solutions may be the same in each electrode reservoir. I am not sure of the logic for why this is, however. I h...
by dhkwak
Mon Feb 01, 2010 7:52 pm
Forum: Molecular Biology
Topic: Running SDS PAGE for the first time!
Replies: 9
Views: 8172

Running SDS PAGE for the first time!

Hey everyone. I'm new to this board and joined in hopes of learning various lab techniques. I am running SDS PAGE shortly and had a few quick questions: Is a running buffer and loading buffer the same thing? If so, are they used as cathode and anode buffers? I have read that cathode and anode buffer...