Search found 440 matches

by jonmoulton
Wed Nov 06, 2013 8:00 pm
Forum: General Discussion
Topic: Please help me decide which microscope to buy
Replies: 2
Views: 5117

Re: Please help me decide which microscope to buy

An inverted scope has the optics below the stage, allowing you to get close (plastic-thickness) to the cells without dumping the objective into the culture media. This will probably limit the maximum magnification you can use, because the short focal length of a very high-power objective may end up ...
by jonmoulton
Mon Nov 04, 2013 8:51 pm
Forum: Molecular Biology
Topic: Review of exon skipping for human therapeutics
Replies: 0
Views: 2912

Review of exon skipping for human therapeutics

Mullard A. Make or break for first splice-modulating agents. Nat Rev Drug Discov. 2013 Oct 31;12(11):813-5. doi: 10.1038/nrd4151. No abstract available. http://www.nature.com/nrd/journal/v12/n11/full/nrd4151.html This is an interest of mine, as my wife was involved in development of the compound Sar...
by jonmoulton
Mon Nov 04, 2013 4:07 pm
Forum: General Discussion
Topic: How do i know if a reaction is endothermic or exothermic?
Replies: 1
Views: 11978

Re: How do i know if a reaction is endothermic or exothermic?

Change in enthalpy = sum of bond energies of reactants - sum of energies of products = Total input energy – Total output energy From: Enthalpy Changes in Chemical Reactions m This reverses the sign of the enthalpy. Enthalpy Before: 2(340) = 680 Enthalpy After: 498 Change in Enthalpy: 680 - 498= 182
by jonmoulton
Tue Oct 29, 2013 10:03 pm
Forum: Cell Biology
Topic: Please help me figure this out
Replies: 4
Views: 4839

Re: Please help me figure this out

Look up these terms in your textbook's glossary & index: codon, frameshift. If that doesn't explain it clearly enough to make sense, try searching the terms in wikipedia. Then go back to the question again.
by jonmoulton
Tue Oct 29, 2013 3:14 pm
Forum: Genetics
Topic: Does complementary DNA include only coding sequences ?
Replies: 4
Views: 6416

Re: Does complementary DNA include only coding sequences ?

Right - another technique, like RACE, is usually needed to extend the sequence across UTRs. If the primers are designed against genomic sequence, they can include UTR sequence, but are unlikely to capture the entire UTRs.
by jonmoulton
Mon Oct 28, 2013 3:46 pm
Forum: Genetics
Topic: Does complementary DNA include only coding sequences ?
Replies: 4
Views: 6416

Re: Does complementary DNA include only coding sequences ?

cDNA can contain 5' and/or 3' UTR sequence but not introns.
by jonmoulton
Tue Sep 03, 2013 4:29 pm
Forum: Cell Biology
Topic: prokartotes
Replies: 4
Views: 7252

Re: prokartotes

There is also the thylakoid membrane of a cyanobacterium which might qualify, but whether or not to call that an organelle would likely ignite semantic argument.
by jonmoulton
Tue Aug 20, 2013 2:55 pm
Forum: General Discussion
Topic: lichens
Replies: 3
Views: 2664

Re: lichens

Lichens are not an organism, they are a community. Their component organisms are be classified as organisms, but a community would not be classified as an organism.
by jonmoulton
Mon Aug 19, 2013 4:37 pm
Forum: General Discussion
Topic: Organism that create or supress water
Replies: 10
Views: 8723

Re: Organism that create or supress water

Plants are tricky -- they produce water by respiration but use water during photosynthesis. Burning the forests would not make us drown due to changes in metabolic water flux, but might make us drown when it rains and the hillsides slide into the valleys (trees and bushes stabilize the sloping soil).
by jonmoulton
Mon Aug 12, 2013 4:32 pm
Forum: General Discussion
Topic: Organism that create or supress water
Replies: 10
Views: 8723

Re: Organism that create or supress water

"...are there ever circumstances in which we (or any animal) make more than we drink?" Yes, our water output is on average greater than our water input; we make water. However, as Jack points out, respiration makes a minor addition of water to the flow of water through our system. Plants, ...
by jonmoulton
Fri Aug 09, 2013 7:45 pm
Forum: Genetics
Topic: What causes dominance between alleles?
Replies: 2
Views: 10863

Re: What causes dominance between alleles?

This is not the only way that dominance and recessiveness can happen. But this is a good way to illustrate once possibility (and a fairly common one). When you have two healthy alleles for a particular gene, one copy on a maternal and one on the corresponding paternal chromosome (and excluding the s...
by jonmoulton
Wed Aug 07, 2013 9:54 pm
Forum: General Discussion
Topic: Organism that create or supress water
Replies: 10
Views: 8723

Re: Organism that create or supress water

Look at the equation for respiration. We all create water from oxygen and sugar. However, it would take a very long time to respire a lake's worth of water.