Search found 440 matches

by jonmoulton
Wed May 21, 2008 8:39 pm
Forum: General Discussion
Topic: Hutchinson-Gilford Progeria Syndrome
Replies: 1
Views: 1974

Re: Hutchinson-Gilford Progeria Syndrome

Here are a few references describing research into HGPG using cell culture studies and oligo-based redirection of the splicing of lamin-A. Scaffidi P, Misteli T. Lamin A-dependent nuclear defects in human aging. Science. 2006 May 19;312(5776):1059-63. Epub 2006 Apr 27. Scaffidi P, Misteli T. Reversa...
by jonmoulton
Tue May 20, 2008 3:30 pm
Forum: Cell Biology
Topic: Do all cells have the ability to perform photosynthesis?
Replies: 11
Views: 16437

Re: Do all cells have the ability to perform photosynthesis?

@deostroll: you wrote "For photosynthesis cells need components called cholroplasts." However, cyanobacteria carry out photosynthesis without choroplasts.
by jonmoulton
Mon Apr 21, 2008 11:42 pm
Forum: Genetics
Topic: New theory... ?
Replies: 5
Views: 2731

Re: New theory... ?

I didn't see the documentary, but there is a layer of gene expression regulation that is controlled by RNA, especially by microRNAs (miRNA), and that is a fairly recent discovery. Is that what you are referring to?
by jonmoulton
Fri Apr 18, 2008 3:34 pm
Forum: Molecular Biology
Topic: alternative splicing
Replies: 1
Views: 2615

Re: alternative splicing

Hi Noy K, Alternative splicing is regulated by a number of factors. One is the strength of the snRNP binding sites, the U1 and U2 sites of the splice donor and splice receptor of the major splicosome. Non-consensus splice sites bind snRNPs less strongly and so are less likely to dominate splicing. V...
by jonmoulton
Fri Apr 04, 2008 9:29 pm
Forum: Molecular Biology
Topic: animal kingdom
Replies: 2
Views: 2602

Re: animal kingdom

This is a new resource and has a long way to develop, but it might be useful.

http://www.eol.org/
by jonmoulton
Mon Mar 17, 2008 6:59 pm
Forum: Genetics
Topic: knockout gene
Replies: 1
Views: 1676

Re: knockout gene

Try this one, it's not a bad place to get started.

http://en.wikipedia.org/wiki/Knockout_gene
by jonmoulton
Tue Mar 04, 2008 8:42 pm
Forum: General Discussion
Topic: DIG system
Replies: 1
Views: 1536

Re: DIG system

Sounds like a densitometry problem, but I wouldn't be terribly confident of quantitative reproducibility with a chromogenic system like most digoxigenin-based assays. The usual quick-and-dirty approach is to scan the blot and measure the amount of blackness of each dot/band, using software like NIH ...
by jonmoulton
Thu Feb 21, 2008 5:55 pm
Forum: Molecular Biology
Topic: Can Western blot be completely replaced by ELISA?
Replies: 2
Views: 2326

Re: Can Western blot be completely replaced by ELISA?

Western gives size information about the analyte, while ELISA gives only concentration information. It's like trying to replace Northerns with RT-PCR: OK for some applications, but if you need to know the size of the analyte you need to do the electrophoretic step on the original sample (and not jus...