Search found 440 matches

by jonmoulton
Wed Jun 11, 2014 2:08 pm
Forum: Cell Biology
Topic: How did endosymbionts coordinate replication?
Replies: 12
Views: 30701

Re: How did endosymbionts coordinate replication?

Regarding the regulation of replication of mitochondria, this paper discusses an influence of tumor necrosis factor alpha on mitochondrial replication while exploring the effect of adenosine on that regulatory system. m Kalogeris TJ, Baines C, Korthuis RJ (2014) Adenosine Prevents TNFα-Induced Decre...
by jonmoulton
Wed May 07, 2014 6:06 pm
Forum: General Discussion
Topic: Elastic cytoplasm film.
Replies: 4
Views: 8019

Re: Elastic cytoplasm film.

It sounds like a black lipid membrane system is the sort of system you are looking for. However, the membranes are fairly unstable, so I doubt you could set one up that would last for months.

http://en.wikipedia.org/wiki/Model_lipid_bilayer
by jonmoulton
Fri Apr 11, 2014 2:47 pm
Forum: Genetics
Topic: DNA testing times
Replies: 2
Views: 6055

I'm not doing this stuff these days, so you should find a better source for confirmation. In general the process involves: (1) sample preparation (2) running an assay (3) analysis The time required will depend on what sort of information you need and therefore what assay you would need to perform, w...
by jonmoulton
Fri Apr 11, 2014 2:36 pm
Forum: General Discussion
Topic: Elastic cytoplasm film.
Replies: 4
Views: 8019

Re: Elastic cytoplasm film.

I don't know what a "cytoplasm film" would be. A black membrane system comes to mind, in which a planar phospholipid bilayer is formed by painting the phospholipids across a small aperture in a rigid sheet separating two chambers containing aqueous solutions. Such a system cannot withstand...
by jonmoulton
Fri Apr 04, 2014 2:59 pm
Forum: Cell Biology
Topic: diffusion
Replies: 2
Views: 5698

Re: diffusion

Bigger molecules diffuse more slowly.
by jonmoulton
Fri Mar 07, 2014 5:57 pm
Forum: Molecular Biology
Topic: Gene knockdowns in the wasp Nasonia vitripennis
Replies: 0
Views: 4767

Gene knockdowns in the wasp Nasonia vitripennis

This paper describes gene knockdowns in the wasp Nasonia vitripennis by Morpholino antisense oligos, delivered by microinjection. Dual mode of embryonic development is highlighted by expression and function of Nasonia pair-rule genes. Rosenberg MI, Brent AE, Payre F, Desplan C. eLife. 2014;3:e01440 ...
by jonmoulton
Thu Mar 06, 2014 4:19 pm
Forum: Molecular Biology
Topic: Molecular medicine: antisense
Replies: 0
Views: 2681

Molecular medicine: antisense

The human side of clinical trials: these are articles about efforts to advocate for a new medicine. Here are a few articles on Duchenne muscular dystrophy and eteplirsen, a Morpholino antisense drug currently in clinical trials. http://www.huffingtonpost.com/tracy-seckler/eteplirsen-duchenne_b_49048...
by jonmoulton
Mon Feb 24, 2014 4:12 pm
Forum: General Discussion
Topic: What is the most promising field of study right now
Replies: 3
Views: 7108

Re: What is the most promising field of study right now

Regarding pharmacy, you might be more interested in pharmacology or medicinal chemistry. Molecular biology will also impact future medicinal chemistry. I suggest focusing toward chemistry, biochem and molecular biology - it sounds like you have a taste for that. Biological chemistry will be in deman...
by jonmoulton
Wed Feb 12, 2014 4:12 pm
Forum: Cell Biology
Topic: mitosis and miosis
Replies: 2
Views: 8100

Re: mitosis and miosis

Wikipedia should be a good source for an explanation.
by jonmoulton
Thu Feb 06, 2014 4:17 pm
Forum: Molecular Biology
Topic: Is this true?
Replies: 2
Views: 2660

Re: Is this true?

What level of detail in our understanding are you looking for? One could reasonably argue that we don't understand how any process works; if you keep probing deeper, you uncover uncertainties.
by jonmoulton
Tue Jan 28, 2014 3:53 pm
Forum: Cell Biology
Topic: centrifugation of hemocytes
Replies: 5
Views: 11445

Re: centrifugation of hemocytes

Before you wash the cells, can you see any pellet? Wash gently, add your final PBS and pipet to resuspend. Your protocol sounds good to me.