Search found 440 matches

by jonmoulton
Mon Sep 29, 2014 3:03 pm
Forum: Molecular Biology
Topic: translation from DNA
Replies: 5
Views: 17840

Re: translation from DNA

That's a reasonable idea. I can imagine a ribosome directly binding to DNA to translate the DNA codons directly into protein. We can't really tell why that didn't happen, as much of the early development of life occurred far in the past and left no evidence of the steps in the process; we must infer...
by jonmoulton
Mon Sep 15, 2014 3:04 pm
Forum: Cell Biology
Topic: Any ideas for vaccine development or anti-viral drugs???
Replies: 1
Views: 28226

Re: Any ideas for vaccine development or anti-viral drugs???

Happens I have this handy. Following are citations of Ebola or Marburg filovirus drug development using Morpholino antisense oligos. Enjoy. -------------------------------------- Enterlein S, Warfield KL, Swenson DL, Stein DA, Smith JL, Gamble CS, Kroeker AD, Iversen PL, Bavari S, Muhlberger E. VP35...
by jonmoulton
Mon Sep 08, 2014 5:27 pm
Forum: Cell Biology
Topic: mRNA vs. cell reporter assay
Replies: 3
Views: 9212

If the cells autofluoresce, this could confound interpretation of the GTP signal. if you are looking for downregulation of transcription, it takes a while for preexisting GPR to degrade but the mRNA signal is likely to disappear fairly rapidly once transcription stops. For upregulation, mRNA will re...
by jonmoulton
Mon Sep 08, 2014 5:23 pm
Forum: Genetics
Topic: Mitochondrial DNA
Replies: 6
Views: 15245

Re: Mitochondrial DNA

There are genetic diseases caused by defects carried on the mitochondrial chromosome that can have clear phenotypic consequences. http://www.umdf.org/site/c.8qKOJ0MvF7LUG/b.7934627/k.3711/What_is_Mitochondrial_Disease.htm
by jonmoulton
Thu Sep 04, 2014 3:04 pm
Forum: Genetics
Topic: Chromatin
Replies: 2
Views: 9119

Re: Chromatin

Before replication there were 23 pairs of molecules, 23 of maternal origin and 23 of paternal origin, plus the maternally-contributed mitochondrial chromosome (which I'll ignore for the rest of this post). Counting chromosomal centromeres, there are 46 centromeres total. After replication there are ...
by jonmoulton
Wed Sep 03, 2014 3:06 pm
Forum: Genetics
Topic: Chromosome Differences
Replies: 5
Views: 8143

Re: Chromosome Differences

The DNA is never connected into a long single molecule in human cells. The DNA remains distributed into the 23 pairs of chromosomes (plus the mitochondrial chromosome), each single DNA molecules complexed with proteins. Each chromosome is replicated and then the chromosomes separate as a collection ...
by jonmoulton
Thu Aug 21, 2014 3:06 pm
Forum: Molecular Biology
Topic: The effect of low temperature on Ascorbic Acid
Replies: 4
Views: 7193

Re: The effect of low temperature on Ascorbic Acid

Might enzymes be present in the orange even if none are added experimentally? If enzymes are present, might they affect the chemistry?
by jonmoulton
Fri Aug 15, 2014 3:32 pm
Forum: General Discussion
Topic: The most boring tasks in Biology
Replies: 1
Views: 5135

Re: The most boring tasks in Biology

Counting cyanobacteria using a hemocytometer on a fluorescent microscope was boring. I was measuring the change in photosynthetic characteristics after changes in illumination at different iron concentrations. This involved lots of time points where I needed the cell density in the culture, so I spe...
by jonmoulton
Thu Jul 24, 2014 2:48 pm
Forum: General Discussion
Topic: suggest me a microscope
Replies: 1
Views: 2792

Re: suggest me a microscope

Zeiss, Nikon and Leica are good names in microscopy. If you can afford them, start there. If you are after lots of magnification, I'd look for a 100X objective and 10x ocular configuration. There is no point in more magnification, you are reaching the limit of resolution of an optical scope. Conside...
by jonmoulton
Wed Jul 02, 2014 2:38 pm
Forum: Cell Biology
Topic: What does "deficient trafficking" means
Replies: 2
Views: 7008

Re: What does "deficient trafficking" means

You can replace: "deficient trafficking to the..." with: "not enough transport to the...". Without reading the paper I can't be sure of this. Based on your post, it sounds like the protein if interest is usually sent to the lysosome, but because it is misfolded it is tagged with ...
by jonmoulton
Fri Jun 20, 2014 2:45 pm
Forum: Molecular Biology
Topic: 2.5 M glycine crystallized
Replies: 1
Views: 6946

Re: 2.5 M glycine crystallized

It's normal for some things to crystallize out of aqueous solution, especially at reduced temperatures. Apparently glycine is such a molecule. Storage at room temperature might prevent the crystallization, but you might also get microbial growth if there are any critters in there. Try gently warming...