Search found 585 matches

by sdekivit
Tue Feb 20, 2007 8:54 pm
Forum: Cell Biology
Topic: Receptor
Replies: 11
Views: 8443

Dr.Stein wrote:
sdekivit wrote:receptor up- and downregulation ;)

Aha! Nice to hear this term again after looong times ;)


very nice terms :D i investigated these phenomena on rat ileum in relation with mast cell degranulation.
by sdekivit
Sun Feb 18, 2007 9:39 pm
Forum: Physiology
Topic: Cyclic AMP
Replies: 2
Views: 5551

look for the signal transduction cascade beyond cAMP and find out what the effects will be.
by sdekivit
Sun Feb 18, 2007 9:36 pm
Forum: Cell Biology
Topic: RNA
Replies: 18
Views: 9054

After a certain amount of time the message degrades into its component nucleotides, usually with the assistance of RNA polymerases. RNA polymerases create the mRNA. It is degraded in a multiprotein structure called an exosome, which is similar to the proteosome that degrades proteins in 3d structur...
by sdekivit
Sat Feb 17, 2007 4:02 pm
Forum: Molecular Biology
Topic: Protein-biomolecules
Replies: 4
Views: 7996

next time don't make someones homework ;)
by sdekivit
Sat Feb 17, 2007 4:01 pm
Forum: Cell Biology
Topic: Receptor
Replies: 11
Views: 8443

receptor up- and downregulation ;)
by sdekivit
Sat Feb 17, 2007 10:32 am
Forum: Molecular Biology
Topic: RNAi uses
Replies: 5
Views: 3548

you can do a homolgy BLAST ;)
by sdekivit
Sat Feb 17, 2007 10:30 am
Forum: General Discussion
Topic: general chemistry
Replies: 1
Views: 1652

the pOH of the solution is 4, thus the pH = 10 -->A: water has a pH = 7 --> every pH-step is 10 times a difference H(+) (log-units --> log 10 =, log 100 = 2 and so on): true. --> B: pH is indeed 10 --> C: true: three pH-units more basic is equal to 1000 tim es OH(-)ions more. --> D: At pH = 10 [H(+)...
by sdekivit
Thu Feb 15, 2007 10:14 pm
Forum: Molecular Biology
Topic: RNAi uses
Replies: 5
Views: 3548

you always need to be cautiopus no other sequences with high sequence homology are also inhibited by the RNAi molecule. That's why you always test multiple RNAi-molecules on there specificity. But your idea about knocking down the ghrelin gene is not wrong, but you need to take into acount how you'r...
by sdekivit
Thu Feb 08, 2007 8:16 pm
Forum: Cell Biology
Topic: Krebs Cycle
Replies: 14
Views: 7668

don't understand the question.
by sdekivit
Sun Feb 04, 2007 9:12 am
Forum: Molecular Biology
Topic: DNA mismatch repair
Replies: 3
Views: 2591

MrMistery wrote:ok thanks for the answer. Methylation of adenine to avoid cleavage? good to know. Cause i just read that "the DNA is methylated" in that situation...


for the mismatch reapir system you need to remember the CpG-islands that can be methylated :)
by sdekivit
Sat Feb 03, 2007 9:02 pm
Forum: Molecular Biology
Topic: DNA mismatch repair
Replies: 3
Views: 2591

according to 'Biochemistry' 5th edition by Berg et al it is the cytosine that is methylated on the C-5 by methyl transferases. I am also familiar with methylated adenine to avoid cleavage by restriction endonucleases.
by sdekivit
Thu Feb 01, 2007 8:30 pm
Forum: Cell Biology
Topic: lipase enzyme
Replies: 4
Views: 2483

probably michaelis-menten ? but when i determine enzyme activities, i draw an absorption curve with a spectrophotometric assay.