Search found 585 matches

by sdekivit
Sat Jul 14, 2007 6:47 pm
Forum: Molecular Biology
Topic: RNA extraction Kit vs Trizol
Replies: 7
Views: 7951

what are the specifications of the kit. Maybe you should compare the experimental details of both experiments to descide what is going on here.
by sdekivit
Fri Jul 13, 2007 8:02 am
Forum: Genetics
Topic: Karyotyping and Probing
Replies: 1
Views: 2063

i would try a FISH experiment. Since a Robertsonian translocation is a dicentric fusion and the acentric parts fragment will be lost, you should be able to detect a Robertsonian translocation using probes for ed 14 & 21 (you'll find only 1 strand of both connected to each other)
by sdekivit
Thu Jul 12, 2007 8:40 am
Forum: Human Biology
Topic: movement of blod into veins
Replies: 1
Views: 2340

my knowledge in anatomy isn't that great, but i thought that the veins run through the muscular part of the diaphragm, so when the diaphragm contracts, the veins will be compressed
by sdekivit
Sun May 27, 2007 8:11 am
Forum: Molecular Biology
Topic: Which one is the correct answer?
Replies: 18
Views: 7972

the answer is indeed C, because this primer set binds both the upperstrand (1 primer is complentary to the 5' side of the upper bold strand) and the lower strand (1 primer is complementary to the 3'side of the lower bold sequence)
by sdekivit
Sat May 19, 2007 8:40 am
Forum: Physiology
Topic: REALLY NEED ANSWER
Replies: 2
Views: 3891

this can be caused by an (allergic) anaphylactic shock. As you probably know: opening of the cappillary sphinjcters tremendously lower the blood pressure so that is the cause of death eventually.
by sdekivit
Sat May 19, 2007 8:38 am
Forum: Physiology
Topic: How does immune system distinguish "self" and &quo
Replies: 3
Views: 7070

not all are destroyed !!! some go into anergy and can indeed cause auto-immune disorders when stimulated in the periphery.
by sdekivit
Sat Apr 07, 2007 10:26 am
Forum: Genetics
Topic: Quadhybrid/Four Traits Genotype Ratio
Replies: 2
Views: 5532

use basic mathematics. what will you cross: AABBCCDD x aabbccdd ?
by sdekivit
Sat Apr 07, 2007 10:05 am
Forum: Molecular Biology
Topic: How do cell sense DNA damage?
Replies: 1
Views: 2091

look for the p53 mechanism with molecules like ATM in the signal transduction pathway leading to induction of p21 and thus cell cycle arrest or apoptosis.
by sdekivit
Sat Apr 07, 2007 10:02 am
Forum: Cell Biology
Topic: chromatography
Replies: 1
Views: 1486

it can be a slight mistake in you mobiule phase (slightly more polar, since the Rf-values are higher if you use normal phase).

Baed on your results, i would say Rf = 0,76 = DNA and Rf = 0,44 = RNA. Probably the other fraction is unremoved denatured protein.
by sdekivit
Sat Apr 07, 2007 9:57 am
Forum: Cell Biology
Topic: ELISA Hormone Assay
Replies: 5
Views: 10549

expand your calibration curve with higher testosterone concentrations and/or greater concentration steps. And then interpolate.
by sdekivit
Sat Apr 07, 2007 9:55 am
Forum: Cell Biology
Topic: confused about DNA v RNA
Replies: 1
Views: 1381

look in your textbook: the chemical structure of RNA en DNA is different en that's why RNA is more susceptible for degradation.

Beside this: there are different RNA moloecules with differing functions and synthesis enzymes.
by sdekivit
Tue Feb 27, 2007 7:53 am
Forum: Cell Biology
Topic: fats and energy
Replies: 8
Views: 8181

how are fats stored in cells ? remember cells are in a polar environment and fats are hydrofobic.