Search found 81 matches

by roniadam
Thu Apr 03, 2008 2:26 pm
Forum: Molecular Biology
Topic: PCR primer design
Replies: 2
Views: 1442

PCR primer design

Hi I want to design a primer for my sequence and i got one primer compatible with one end (5') and the other is compatible with the middle of my sequence not the 3' end. My question is this ok if I want to amplify this sequence. What about the other parth that is located behind the right primer? sec...
by roniadam
Wed Apr 02, 2008 11:35 pm
Forum: Molecular Biology
Topic: Amplification of 7 kb fragment by PCR
Replies: 2
Views: 2382

Thanks Canalon. You are usually a helpful man.
by roniadam
Wed Apr 02, 2008 3:36 pm
Forum: Molecular Biology
Topic: Amplification of 7 kb fragment by PCR
Replies: 2
Views: 2382

Amplification of 7 kb fragment by PCR

Hi everyone:

I need to know what type of PCR is suitable for amplifying a DNA fragment that is 7 kbp and why ?
by roniadam
Wed Mar 26, 2008 1:32 pm
Forum: Molecular Biology
Topic: DNA insert preparation for cloning
Replies: 1
Views: 982

DNA insert preparation for cloning

Hi all:

I have a fragment of DNA (CDS of sheep complement factor B) and I would like to insert this sequence into a vector... How can I do this theoritically step by step? can any one explain to me?
by roniadam
Sat Mar 22, 2008 3:22 pm
Forum: Molecular Biology
Topic: Regulatory elements of genes
Replies: 3
Views: 3844

Regulatory elements of genes

Hi Everyone: Can any person tell me if there are free software programs to predict the regulatory elements of genes? Second question : what i know about regulatory elements of gene is that they include Promoter, Poly A signal and Splice site. Is there any regulatory elements other than these?? Thank...
by roniadam
Thu Mar 20, 2008 1:51 pm
Forum: Molecular Biology
Topic: Protein expression
Replies: 1
Views: 1258

Protein expression

HI
Can anyone tell me why is the frame of the inserted DNA important in bacterial expression system while it is not important for mammalian and baculovirus system?
by roniadam
Mon Mar 10, 2008 2:25 pm
Forum: Molecular Biology
Topic: Regulatory elements of complement factor B
Replies: 0
Views: 857

Regulatory elements of complement factor B

Hi All

I need to know what does regulatory elements of a gene mean???
can anyone explain what type of regulatory elements does the complement factor B gene have?
by roniadam
Sun Mar 09, 2008 6:23 am
Forum: Molecular Biology
Topic: Run off assay for in vitro transcription
Replies: 0
Views: 2409

Run off assay for in vitro transcription

HI

What is the detailed procedure for in vitro transcription of drosophila engrailed gene?
Can any one suggets a step by step book to do this experiment?
by roniadam
Tue Mar 04, 2008 2:28 pm
Forum: Bioinformatics
Topic: Which software to be used???
Replies: 2
Views: 3583

Which software to be used???

Hi everyone:

I have a DNA fragment for analysis. Through BLAST ncbi, I identified the gene of the DNA as complement factor B. My question is what software I should use to identify the structure of the gene?
by roniadam
Fri Feb 29, 2008 10:50 am
Forum: Molecular Biology
Topic: To whom working with DNA sequencing
Replies: 3
Views: 1662

Still cannot find the answers
by roniadam
Wed Feb 27, 2008 11:32 pm
Forum: Molecular Biology
Topic: To whom working with DNA sequencing
Replies: 3
Views: 1662

To whom working with DNA sequencing

1. Would a primer extension reaction in DNA sequencing work using an RNA polymerase? any one can explain this point please? 2. When the products of a DNA sequencing reaction are separated by electrophoresis, why is it normally only possible to read about 500 to 800 bases, yet the sequencing reaction...
by roniadam
Mon Feb 25, 2008 10:24 am
Forum: Molecular Biology
Topic: DNA sequencing
Replies: 3
Views: 1878

DNA sequencing

Why is it harder to sequence CG rich DNA sequences compared to AT rich ?