Search found 18 matches

by citroenboom
Tue Jul 17, 2012 6:59 am
Forum: Microbiology
Topic: Fungus growing on agar plates. contamination??
Replies: 17
Views: 23453

In my lab the cold room is a nice source of some black fungi (we need to clean). Only carefully closed bags are good enough to keep it out. So I think it is air and water droplets in in the air. Just be carefull when pouring and storing. Pouring next to the flame and storing upside down in a bag in ...
by citroenboom
Thu Jun 14, 2012 1:03 pm
Forum: Molecular Biology
Topic: separation problem with GelRed
Replies: 12
Views: 34281

Just saw the gels. Type of marker makes a difference. If bands are close, the gel is easily overloaded. Overall, 0.5x buffer does not help. GelRed is out of here I think. Markers keep looking bad ...
by citroenboom
Thu Jun 14, 2012 11:50 am
Forum: Microbiology
Topic: Growing bacteria in the laboratory
Replies: 7
Views: 5546

They grow the in the same way as in nature.
They eat (take up) nutrients, produce proteins, replicate their DNA and divide.
Very efficient.
by citroenboom
Thu Jun 14, 2012 11:44 am
Forum: Molecular Biology
Topic: separation problem with GelRed
Replies: 12
Views: 34281

Re: separation problem with GelRed

Hi there, after few tests, the similar problem still exist, especially for the ladder. my lab uses both 1% and 2% gel. 1x TAE for dissolving and also running. 100v for 50mins. I have tried using low voltage and higher voltage as well, yet the output still the same. Any suggestion or advise? Thank y...
by citroenboom
Thu Jun 14, 2012 11:42 am
Forum: Molecular Biology
Topic: separation problem with GelRed
Replies: 12
Views: 34281

We try this 0.5 x buffer protocol on the moment.
Mixing (homogenizing) of the marker in the well also improved results.
When done I will try to put a link to the gel pictures.
by citroenboom
Wed Jun 13, 2012 2:46 pm
Forum: Molecular Biology
Topic: restriction reaction on agarose gel slices
Replies: 2
Views: 2673

Re: restriction reaction on agarose gel slices

Why use gel extraction? Often EtBr seems to inhibit ligation. You could use PCR purification kits. Much faster!
Or otherwise go for the good old phenol extraction (Sambrook). Quick, but dirty :)