Search found 5 matches

by evamik
Thu Jun 07, 2012 8:02 am
Forum: Molecular Biology
Topic: Protein expression but no mRNA
Replies: 10
Views: 12454

Re: Protein expression but no mRNA

Sorry for not responding sooner. The protein is indeed present in serum. A strong band is detected both in patients and in healthy controls. The molecular weight in serum is the same as seen in cell lysates. Maybe, the protein is simply bound to cell surface receptors?!? But when I do fractionation ...
by evamik
Wed Feb 22, 2012 2:01 pm
Forum: Molecular Biology
Topic: Protein expression but no mRNA
Replies: 10
Views: 12454

Good point!! But the cells I work with are primary cells (lymphocytes isolated directly from fresh blood). The leukemia I work on is characterized by a SLOW progression only partly due to proliferation and even more due to inhibited apoptosis. Is endocytosis a completely unthinkable (and maybe stupi...
by evamik
Wed Feb 22, 2012 1:52 pm
Forum: Molecular Biology
Topic: Protein expression but no mRNA
Replies: 10
Views: 12454

Re: Protein expression but no mRNA

Absolutely! And the patient sampes tested are clearly positive.
by evamik
Tue Feb 21, 2012 7:28 am
Forum: Molecular Biology
Topic: Protein expression but no mRNA
Replies: 10
Views: 12454

Re: Protein expression but no mRNA

Good point, but the detected protein is a single band of the expected size so I can not really believe that it is unspecific binding.
by evamik
Fri Feb 17, 2012 8:18 am
Forum: Molecular Biology
Topic: Protein expression but no mRNA
Replies: 10
Views: 12454

Protein expression but no mRNA

I am looking into the expression of a certain gene that is clearly overexpressed both at the gene (RT-PCR) and protein (Western blot) levels in some malignancies. However, when I test healthy controls, they are clearly positive in Western blot but completely negative in RT-PCR. I have used several d...