Search found 2 matches

by Jemi
Tue Feb 21, 2012 3:30 pm
Forum: Molecular Biology
Topic: Microparticles and flow cytometry
Replies: 5
Views: 4509

Microparticles and flow cytometry

Hi, I've been given several flow cytometry profiles to analyse for a piece of work and I'm slightly confused about one of them. http://farm8.staticflickr.com/7199/6916515519_17139005f3_m.jpg So far I've worked out that it shows microparticles (I think!) and I know that more side scatter means a more...
by Jemi
Wed Feb 15, 2012 8:11 pm
Forum: Molecular Biology
Topic: Designing primers to clone into expression vector
Replies: 1
Views: 3022

Designing primers to clone into expression vector

Hi, I'm just doing a piece of work at the moment and I'm a little stuck. I've got a DNA ligase sequence and I've created primers for it to mutate two residues but now I have to design two outer primers to clone the gene into a pET expression vector and I have no idea how! I've pretty much been stari...