Search found 2 matches

by wetheril
Fri Mar 30, 2007 8:48 pm
Forum: Molecular Biology
Topic: Immunohistochemistry HELP--paraffin vs frozen sections
Replies: 0
Views: 3287

Immunohistochemistry HELP--paraffin vs frozen sections

Hi, I'm new to immunohistochemistry slide staining, and I'm having problems getting the staining to show correctly. So far, I've been staining lymph node tissue for CD62L (L-selectin) and CD3, both of which are supposed to be quite abundant on the surface of regular T cells in the lymph node (this i...
by wetheril
Tue Dec 12, 2006 12:26 am
Forum: Molecular Biology
Topic: His-tag on primer?
Replies: 4
Views: 7442

His-tag on primer?

Hi, I ventured into this forum because this is the first semester I started to take biotechnology classes, and this place seems like the perfect place to find some answers to questions. I want to design a primer for PCR that will create DNA that encodes for a his-tagged protein (the DNA will be tran...