Search found 2 matches

by ruanhocon
Fri Mar 16, 2007 2:19 pm
Forum: Molecular Biology
Topic: DNA extraction methods
Replies: 1
Views: 1548

DNA extraction methods

Dear all,

I need to isolate DNA from plaque samples containing Gram + and Gram - bacteria. I am using now FastDNA spin kit for soil, but it did not work well for me. I did not see the DNA band on agarose gel. Could you recommend any other methods for isolating DNA?

Thank you,
by ruanhocon
Fri Dec 08, 2006 10:49 am
Forum: Molecular Biology
Topic: LH-PCR and TRFLP analysis
Replies: 0
Views: 1190

LH-PCR and TRFLP analysis

Dear all,

Is there anyone here working on LH-PCR and TRFLP? I want to ask how you can normalize the result? I used genemapper v. 3.7 and everytime I repeat the experiment, the results were slightly different ( for instance 345.2; 345.89, ect).

Thank you so much,