Search found 2 matches

by silvie
Sat Dec 04, 2004 10:27 pm
Forum: Cell Biology
Topic: Role of EDTA in protoplast lysis?
Replies: 3
Views: 13584

@biostudent84 : Thank you for your reply. This project is part of my PhD work, which I have just started a few weeks ago in Stuttgart (Germany). I asked that question to nearly all people I know, but nobody seems to know why protoplast lysis works better with EDTA than without. Seems that some metal...
by silvie
Fri Dec 03, 2004 3:18 pm
Forum: Cell Biology
Topic: Role of EDTA in protoplast lysis?
Replies: 3
Views: 13584

Role of EDTA in protoplast lysis?

Hello together! I am isolating vacuoles and for that purpose I first purify protoplast of Arabidopsis leave mesophyll. Afterwards I have to lysate the protoplasts. The lysis buffer contains EDTA. Can anyone tell me what the exact function of EDTA is in protoplast lysis? For certain causes (measureme...