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- Tue Feb 07, 2012 2:57 am
- Forum: Molecular Biology
- Topic: need help with PCR gel
- Replies: 0
- Views: 1550
I made a PCR product of 20ul total, ran 3ul of the product on a 2% agarose pre-cast gel. This is the first picture. There is a nice band at the correct bp size (400bp), but a fainter band is above it. So I wanted to gel purify out my correct band. I ran the 17ul leftover on a poured 2.5% TBE gel, at...