Search found 3 matches
Search found 3 matches • Page 1 of 1
- Wed Nov 29, 2006 6:11 pm
- Forum: Microbiology
- Topic: alternatives to generalized phage transduction?
- Replies: 0
- Views: 1535
I have been trying to transduce my recipent strains with mutations from the donor strain, but I don't get any colonies. I have checked plate concentrations, I have remade my phage lysates and even tried other strains. Even if there are four or five colonies, they don't have the mutation. Does anyone...
- Mon Nov 27, 2006 11:06 pm
- Forum: Molecular Biology
- Topic: Need help interpreting Western Blot results
- Replies: 3
- Views: 4520
I recently did western blots with Pbp2a polyclonal antibodies to detect the presence of protein of interest--penicillin binding protein 2a (Pbp2a). The expected product size is ~75kDa. Goat anti-rabbit peroxidase is my secondary. The following are the problems I encountered. 1. Consistently, I get a...