Search found 3 matches

by schwartz
Fri Jan 19, 2007 4:41 pm
Forum: Molecular Biology
Topic: Need help interpreting Western Blot results
Replies: 3
Views: 4403

hi! thanks for your reply. I will give the CIP a try to see if the bands disappear or not... thanks again.
by schwartz
Wed Nov 29, 2006 6:11 pm
Forum: Microbiology
Topic: alternatives to generalized phage transduction?
Replies: 0
Views: 1488

alternatives to generalized phage transduction?

I have been trying to transduce my recipent strains with mutations from the donor strain, but I don't get any colonies. I have checked plate concentrations, I have remade my phage lysates and even tried other strains. Even if there are four or five colonies, they don't have the mutation. Does anyone...
by schwartz
Mon Nov 27, 2006 11:06 pm
Forum: Molecular Biology
Topic: Need help interpreting Western Blot results
Replies: 3
Views: 4403

Need help interpreting Western Blot results

I recently did western blots with Pbp2a polyclonal antibodies to detect the presence of protein of interest--penicillin binding protein 2a (Pbp2a). The expected product size is ~75kDa. Goat anti-rabbit peroxidase is my secondary. The following are the problems I encountered. 1. Consistently, I get a...