Search found 4 matches

by sims10
Mon Nov 27, 2006 5:09 pm
Forum: Molecular Biology
Topic: Plasmid Restriction Maps
Replies: 4
Views: 6159

ok so I tried solving it...can you check if I'm correct...

so at the top is 5400 bp (BamH1 site)

starting counter-clockwise...

500 bp (Hindlll)
900 bp (BamH1)
2100 bp (Hindlll)
2400 bp (BamH1
by sims10
Mon Nov 27, 2006 12:01 am
Forum: Molecular Biology
Topic: Plasmid Restriction Maps
Replies: 4
Views: 6159

Plasmid Restriction Maps

I'm having problem drawing a plasmid map with the following combination enzymes...

BamH1: 900/1500/3000
Hindlll: 500/1600/3300
BamH1 + Hindlll: 300/400/500/1200/3000

Is there a simple way to solve this?
by sims10
Sun Nov 26, 2006 8:09 pm
Forum: Molecular Biology
Topic: palindromic sequences
Replies: 4
Views: 2360

rob3 wrote:There are two of these as well, maybe they arenot long enough to count.

5' TA 3'


nope it's too short, palindromic sequences are usually four to eight base pairs long
by sims10
Sat Nov 25, 2006 7:23 pm
Forum: Molecular Biology
Topic: palindromic sequences
Replies: 4
Views: 2360

palindromic sequences

5'-CGTAAGTTAACGTCACCTGAGTACGGCTA-3'

there's apparently four palindromic sequences in this fragment. I can only find one... 5' TTAA 3' , can someone tell me where the rest are? I think I may be going blind