Search found 8 matches

by cw11
Tue Nov 29, 2011 2:35 am
Forum: Zoology Discussion
Topic: reptile eggs
Replies: 4
Views: 4954

I think turtles have calcium carbonate in their eggs...
by cw11
Tue Nov 29, 2011 2:31 am
Forum: Zoology Discussion
Topic: Leeches
Replies: 3
Views: 3995

Yes
by cw11
Tue Nov 29, 2011 2:26 am
Forum: Cell Biology
Topic: Some Questions
Replies: 3
Views: 3599

Re: Some Questions

Centromere --> Place where sister chromatids are held in contact
Ethical issue --> Embryonic stem cells
Organisms with a definite nucleus --> Eukaryotes
by cw11
Tue Nov 29, 2011 2:07 am
Forum: Molecular Biology
Topic: Problem with Bgl II Sal digestion
Replies: 5
Views: 3447

Yes, what exactly do you see when you digest with Bgl II and SalI? More fragments than expected, uncut plasmid, plasmid that looks like it was cut only once, nothing...? If one of the first 3, then your restriction sites may be in different places than you think they are...
by cw11
Tue Nov 29, 2011 1:44 am
Forum: Molecular Biology
Topic: Isopropanol, Water, and RNA Stability
Replies: 3
Views: 4300

Re: Isopropanol, Water, and RNA Stability

Ahhh fail! Thanks for your reply! But in that case, it doesn't really actually have to do with the polarity, does it? It has to do with the pKA of the substances involved. Isopropanol, as an alcohol, has a pKa of around 16; water has a pKa of 15.7. So water is very slightly more likely to become OH-...
by cw11
Mon Nov 28, 2011 7:26 pm
Forum: Molecular Biology
Topic: PCR Accuracy?
Replies: 6
Views: 4464

I can think of one case where errors might be more likely to occur - when you have a large number of tandem repeats and strand slippage occurs. But I doubt this is going to be a problem for you. In terms of PCR, if you are sequencing afterwards (as you say you are), the accuracy of most sequencing r...
by cw11
Mon Nov 28, 2011 7:18 pm
Forum: Molecular Biology
Topic: Splicing isoforms
Replies: 4
Views: 5640

I did a quick google search and it seems that isoforms typically refer to proteins, so I believe the correct answer would be (2) - the protein product of already-spliced mRNA.
by cw11
Mon Nov 28, 2011 7:15 pm
Forum: Molecular Biology
Topic: Isopropanol, Water, and RNA Stability
Replies: 3
Views: 4300

Isopropanol, Water, and RNA Stability

I was recently having a discussion with someone who remarked in passing that RNA stability is higher in isopropanol than in H2O. (I am preparing to perform a phenol:chloroform extraction of RNA, and they recommended - for added stability - that I keep the RNA in isopropanol instead of H2O until I'm ...