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by workforfood
Thu Nov 03, 2011 5:17 pm
Forum: Molecular Biology
Topic: Chelex 100
Replies: 6
Views: 16628

The chelex 100 solution is not going to dissolve in its de-ionized water. I have used chelex for quite some time, and I have found that the best extraction results are as follows: 1)spin down a sample to a pellet in a 1.5ml microcentrifuge tube. 2)Decant supernatant 3)Add 100 micro liters of 5% chel...