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by ucsfmed2012
Thu Oct 27, 2011 9:30 am
Forum: Molecular Biology
Topic: hydroxyproline assay to determine collagen content
Replies: 5
Views: 13315

Re: hydroxyproline assay to determine collagen content

Hello Stip, I have an adjusted protocol for intervertebral discs. This assay estimates the amount of hydroxyproline, which has been shown to account for approximately 14% the amino acids in collagen. Materials: Hydroxyproline Standard - 1-5 µg/2 0.001N HCl ml. Buffer-0.5 g citric acid monohydrate, 0...