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- Thu Oct 27, 2011 9:30 am
- Forum: Molecular Biology
- Topic: hydroxyproline assay to determine collagen content
- Replies: 5
- Views: 13315
Hello Stip, I have an adjusted protocol for intervertebral discs. This assay estimates the amount of hydroxyproline, which has been shown to account for approximately 14% the amino acids in collagen. Materials: Hydroxyproline Standard - 1-5 µg/2 0.001N HCl ml. Buffer-0.5 g citric acid monohydrate, 0...