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by francisp
Tue Oct 25, 2011 12:26 am
Forum: Molecular Biology
Topic: Cloning bacteriphage lambda fragments
Replies: 0
Views: 2113

Cloning bacteriphage lambda fragments

We digested bacteriphage lambda with HindIII and EcoRI, ligated the fragments into the vector pHSG398, induced E. coli to take up the vector, plated the E. coli on chloramphenicol resistant plates containing X-gal, then extracted the recombinant plasmid DNA, and finally, double-digested the recombin...