Search found 9 matches

by TheWalkingGlitch
Mon Nov 21, 2011 9:23 pm
Forum: Bioinformatics
Topic: Biological Organization Software
Replies: 5
Views: 19847

.NET is a windows thing (http://en.wikipedia.org/wiki/.NET_Framework) but supposedly there's a linux alternative: http://www.techrepublic.com/article/run ... nu/6310344

Anyway, back on topic, what OP needs is a histology atlas or something. Here's a free one: http://www.visualhistology.com/products ... index.html
by TheWalkingGlitch
Mon Nov 21, 2011 8:30 pm
Forum: Bioinformatics
Topic: antibody/antigen pair with a Kd of 10^-6 M or higher
Replies: 2
Views: 3840

Re: antibody/antigen pair with a Kd of 10^-6 M or higher

Imperfect in what way? For example, if I know of two antibodies that bound to each other with a Kd of 10^-6, I could modify one of them to not bind as well? How could I do that? The Kd thing is kind of important. I know it can be measured but I would prefer an already known pair. Just wondering if a...
by TheWalkingGlitch
Sat Nov 19, 2011 8:48 pm
Forum: Bioinformatics
Topic: antibody/antigen pair with a Kd of 10^-6 M or higher
Replies: 2
Views: 3840

antibody/antigen pair with a Kd of 10^-6 M or higher

Hi, I'm looking for an antibody/antigen pair with a Kd of 10^-6 M or higher, (that is, weaker affinity than that) I was trying to use the protein data bank and could find binding affinity under advanced search, but how do I specify antibody? Also, if anyone happens to know of a pair of molecules tha...
by TheWalkingGlitch
Wed Nov 09, 2011 1:21 am
Forum: Molecular Biology
Topic: Mismatch DNA repair
Replies: 4
Views: 4828

Re: Mismatch DNA repair

Increased efficiency? The processes of strand elongation and primer replacement are spatially/temporally separated, it's faster if 2 polymerases do it at once. Another way of looking at it... proofreading is such an important ability that if one polymerase is defective, the other one still has it. F...
by TheWalkingGlitch
Sun Oct 30, 2011 9:01 pm
Forum: Molecular Biology
Topic: Keeping proteins from washing off the slide
Replies: 5
Views: 3337

OK thanks for your comments. I am trying to see patterns of fluorescence under the fluorescent microscope. I was actually thinking that my actual washing technique might be the problem, however this issue only recently started happening. I've tried washing by squirting with the pipette as well as du...
by TheWalkingGlitch
Sun Oct 30, 2011 2:51 am
Forum: Molecular Biology
Topic: Keeping proteins from washing off the slide
Replies: 5
Views: 3337

Thanks for your response, but I wouldn't want to cover the protein and keep the antibodies from binding.
by TheWalkingGlitch
Fri Oct 28, 2011 8:10 am
Forum: Molecular Biology
Topic: Keeping proteins from washing off the slide
Replies: 5
Views: 3337

Keeping proteins from washing off the slide

I have protein patterns on a bare slide, and I want them to be fluorescent so I incubate them with a primary antibody then a secondary labeled antibody, but the wash afterward is causing the pattern to come off. I've tried washing with PBS as well as DI water. I have also tried various coatings for ...
by TheWalkingGlitch
Thu Oct 20, 2011 2:01 pm
Forum: Microbiology
Topic: "Model organism" for viruses?
Replies: 3
Views: 2153

Re: "Model organism" for viruses?

Thanks, figured it would be some kind of phage because they're not harmful to humans.
by TheWalkingGlitch
Wed Oct 19, 2011 11:08 pm
Forum: Microbiology
Topic: "Model organism" for viruses?
Replies: 3
Views: 2153

"Model organism" for viruses?

When working with bacteria, we use E. coli as a "model organism" and try it first in experiments (because it is cheap, easy to grow, well-studied, etc), and if it works we move on to the bacteria we are really interested in. When working with viruses, is there an analogous "model orga...