Search found 2 matches

by stalinsma
Wed Oct 19, 2011 8:17 pm
Forum: Molecular Biology
Topic: LIC PCR Primer with Signal Peptide?
Replies: 3
Views: 4505

Yep I think I know what you mean. It would appear from the NCBI database that the signal peptide was included as part of the particular protein I searched for, although I don't know why that is (accession number EFF03255). A SignalP analysis showed where the cleavage site was on the submitted amino ...
by stalinsma
Wed Oct 19, 2011 6:03 pm
Forum: Molecular Biology
Topic: LIC PCR Primer with Signal Peptide?
Replies: 3
Views: 4505

LIC PCR Primer with Signal Peptide?

Ha-row, Wondering if anyone here can shed some light on this doozey - I have a target gene sequence I want to amplify using PCR (LIC) but I've been told when designing my primer that I should remove the signal peptide (cleavage site at the 26th amino acid) which corresponds to removing the first 78 ...