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- Thu Sep 08, 2011 11:46 am
- Forum: Molecular Biology
- Topic: Western Blotting of small molecular weight
- Replies: 4
- Views: 9478
Hi, I am working on a project in which I am doing Western Blots to look at the expression of a low molecular weight protein (15 kDa) in various brain regions. I have been using a 0.2 uM nitrocellulose membrane for my transfers and I have been transferring for 1 hour at 100 Volts. I have been getting...