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by yuguh
Wed Aug 17, 2011 2:12 pm
Forum: Molecular Biology
Topic: Enrichment PCR
Replies: 1
Views: 1875

Enrichment PCR

I am doing an enrichment PCR (40 cycles) of a restriction digest product purified with Qiagen PCR clean up kit. I get no band in my experimental lanes, correct band in my positive control and a correct band in my NTC. I have designed different primers switched up all my reagents and I still get the ...