Search found 2 matches

by kafrin313
Thu Aug 11, 2011 10:15 am
Forum: Molecular Biology
Topic: Why use trypsin in cell cultures?
Replies: 1
Views: 17941

Re: Why use trypsin in cell cultures?

Trypsin in principle is considered to be a protease. What a normal protease does is to "cut" the animo-acid bonds by hydrolysis. One vivid example of a similar enzyme is lysozyme; it is located in human saliva and helps us with our food process by hydrolysing the starch that exists in food...
by kafrin313
Thu Aug 11, 2011 9:55 am
Forum: Molecular Biology
Topic: TSH synthesis screening and proper cell lines
Replies: 0
Views: 1828

TSH synthesis screening and proper cell lines

Dear all, I want to perform some ELISA experiments for checking TSH synthesis. I have to use Tαt-1 cell line. Do you have any suggestions of a plan B in case I can't get these cell lines in my hands? Should I search for another cell line with similar properties? Any suggestion is more than welcome. ...