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- Thu Aug 04, 2011 11:06 pm
- Forum: Molecular Biology
- Topic: An interesting PCR smearing...I dont know what to do :(
- Replies: 9
- Views: 14899
Did you ever figure out what caused the smearing? I am having the exact same problem right now - 150 PCR reactions with the same primers and suddenly whole lane smearing. I've changed everything, ordered new primers, even used different pipettes in a different location and I still see the smearing! ...