Search found 6 matches

by aguy
Fri Sep 08, 2017 9:21 pm
Forum: General Discussion
Topic: I have a genome, now what?
Replies: 0
Views: 1814

I have a genome, now what?

Hi, This is not my area so I thought I'd ask. Let's say I take the genome of a plant, say rice, and modify it. Now, I have a new genetic sequence on my computer. Or say I design an entirely new 'rice' genome. How can I go from the sequence to an actual plant? Of course, for the latter, I am assuming...
by aguy
Mon Jul 07, 2014 10:02 pm
Forum: Molecular Biology
Topic: 5' LTR sequece
Replies: 2
Views: 6665

5' LTR sequece

Hi,

I want to check the 5' LTR sequence of my plasmid. I will like to confirm that it is a hybrid 5' LTR and not a wild type HIV-1 5' LTR. Can someone pls guide me on how I can evaluate this?

Best,
John
by aguy
Mon Jul 07, 2014 2:50 am
Forum: Cell Biology
Topic: mRNA vs. cell reporter assay
Replies: 3
Views: 8615

mRNA vs. cell reporter assay

Hi, I have a GFP reporter cell line that drives GFP expression upon activation of a transcription factor. In theory, I can study transcription factor activation via the GFP signal, or by measuring the mRNA of the transcription factor. I wonder what are the pros and cons of each. Can anyone help me f...
by aguy
Sat Mar 09, 2013 5:28 am
Forum: Cell Biology
Topic: glucose content of fbs
Replies: 0
Views: 2215

glucose content of fbs

Hi,

Does anyone know if how much glucose is in fetal bovine serum FBS?

Thanks,
John
by aguy
Thu Jan 24, 2013 11:19 pm
Forum: General Discussion
Topic: embedding cells in paraffin
Replies: 0
Views: 1811

embedding cells in paraffin

Hi,

How can I embed cells in a 6-wells plate into paraffin wax? I cannot detach the cells so this has to be done in situ.

Thanks.
by aguy
Wed Aug 03, 2011 7:53 am
Forum: Molecular Biology
Topic: how to attach dendritic cells for imaging?
Replies: 0
Views: 2160

how to attach dendritic cells for imaging?

Hi, I want to carry out actin staining on human dendritic cells derived from a cell line. The cells are grown in suspension. I am having trouble fixing the cells and subsequent staining because the cells do not attach to a surface (I have tried coating a glass slide with poly L lysine, but it didn't...