Search found 3 matches

by Stip
Wed Aug 03, 2011 7:26 am
Forum: Cell Biology
Topic: NaOH Normality
Replies: 2
Views: 2316

NaOH Normality

Hi there,

Just wondering - If 50uL of 2N NaOH is added to 50uL of H20 what is the final normality of NaOH? I am struggling with my basics. please help
by Stip
Wed Aug 03, 2011 6:15 am
Forum: Molecular Biology
Topic: hydroxyproline assay to determine collagen content
Replies: 5
Views: 13507

Re: hydroxyproline assay to determine collagen content

thanks Jack, here is my protocol: 50ul of test samples containing Hyp (10ug/ml) and aliquots of std hyp (2-20ug) prepared from 10mg/ml stock, this is then hydrolysed with 50ul of 2N NaOH (2N final conc) at 120 degree C for 20 min. To this add 450ul of Chloramine T and oxidise at RT for 25 min. 500ul...
by Stip
Wed Aug 03, 2011 5:55 am
Forum: Molecular Biology
Topic: hydroxyproline assay to determine collagen content
Replies: 5
Views: 13507

hydroxyproline assay to determine collagen content

Hi, I am trying to analyse the hydroxyproline to determine collagen content in rat heart samples, I am following Reddy and Enwemeka 1996 paper but unable to get a proper standard curve, even my blank (water) looks similar to my highest standard concentration (2­­0ug). Not sure where I am going wrong...