Search found 2 matches

by melis
Tue Jul 19, 2011 5:57 pm
Forum: Molecular Biology
Topic: An interesting PCR smearing...I dont know what to do :(
Replies: 9
Views: 14898

Re: An interesting PCR smearing...I dont know what to do :(

Thanks, I reordered all the reagents except primers. So, I finally suspect my primers. After fresh primer usage, if I face with the same problem, I dont know what to do I sterilized my pipettes with UV and tips with autoclave. but I did not do anything with HCl. I shall try it. I have 4 thermocycler...
by melis
Mon Jul 18, 2011 8:55 pm
Forum: Molecular Biology
Topic: An interesting PCR smearing...I dont know what to do :(
Replies: 9
Views: 14898

An interesting PCR smearing...I dont know what to do :(

Hello, My problem is about an interesting smearing of my PCR product. I have done PCR of a plant nuclear DNA with about 6 kb many many times with my specific and well performing primer. About 200 samples of PCR products were obtained without any problem during 3 months i.e. I got sharp PCR bands dur...