Search found 2 matches

by kharsh
Sat Jul 16, 2011 4:34 am
Forum: Molecular Biology
Topic: cloning
Replies: 10
Views: 8014

k wl try the latter option
by kharsh
Fri Jul 15, 2011 11:08 am
Forum: Molecular Biology
Topic: cloning
Replies: 10
Views: 8014

Re: cloning

I am attempting to clone a gene which i have amplified thru PCR. I have added HindIII and NotI sites at its 5' and 3' ends. I have ligated this gene into a vector after double digesting both of them by same HindIII and NotI enzymes. After transforming it into DH5 alpha cells and isolating plasmid i ...