Search found 3 matches

by susi
Fri Jun 30, 2006 8:15 am
Forum: Molecular Biology
Topic: single stranded cDNA storage
Replies: 6
Views: 22542

I am going to use the cDNA directly in a real-time PCR reaction and was told that it's not necessary to make doble stranded cDNA for this purpose. We are going soil-sampling next week, and will return with a lot of samples. I want to quantify many different genes from the same RNA --> cDNA-isolate, ...
by susi
Wed Jun 28, 2006 12:45 pm
Forum: Molecular Biology
Topic: single stranded cDNA storage
Replies: 6
Views: 22542

I'm planning on just doing the first strand reaction (skipping the second strand reaction). Will single stranded cDNA be as stable as double stranded cDNA?
by susi
Wed Jun 28, 2006 11:28 am
Forum: Molecular Biology
Topic: single stranded cDNA storage
Replies: 6
Views: 22542

single stranded cDNA storage

Hi everyone! I am about to start an experiment where I'm going to do a real-time PCR with cDNA as template (real-time RT-PCR). I will start with extracting RNA from soil and do a first strand RT-reaktion. What is the best temperature for storing single stranded cDNA? For how long will it be stable a...