Search found 3 matches

by ludovica
Wed Jul 27, 2011 1:37 pm
Forum: General Discussion
Topic: Help with NFKB
Replies: 0
Views: 1273

Help with NFKB

Hi everyone, I would know if someone is familiar with NFKB because I have to make an experiment with confocal microscopy but I have some doubts....In particular I don't know if I have to use only one antibody (for example anti-NFKB P65) or also anti-NFKB p50 that interacts with p65 to form heterodim...
by ludovica
Wed Jun 15, 2011 10:13 am
Forum: Molecular Biology
Topic: HELP with NFKB!
Replies: 3
Views: 1973

Re: HELP with NFKB!

Thank You very much for the reply.
I'd want detect if the drug has effects on the binding of NFKB with DNA....I have already search possible kits but i have only found ELISAs that requires too much extracts....
by ludovica
Tue Jun 14, 2011 1:43 pm
Forum: Molecular Biology
Topic: HELP with NFKB!
Replies: 3
Views: 1973

HELP with NFKB!

Hello! It would be wonderful if somebody could help me! I have to detect the effect of an immunosuppressant agent on the transcription factor NFKB but not with Western or ELISA.....My problem is that I have a very little amount of cells so it's difficult to have nuclear extract enough for ELISA....I...