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- Mon May 23, 2011 7:21 pm
- Forum: Molecular Biology
- Topic: LIC insertion for dual protein expression
- Replies: 0
- Views: 1336
Dear All, I currently have a gene coding for a two domain protein cloned into an expression vector (pET28b). I would like to express the two domains as separate genes but in the same vector. I though that I could do it by PCR using two primers, one 5'-3' that anneals with the start of the second dom...