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by cpmf752004
Mon May 23, 2011 7:21 pm
Forum: Molecular Biology
Topic: LIC insertion for dual protein expression
Replies: 0
Views: 1293

LIC insertion for dual protein expression

Dear All, I currently have a gene coding for a two domain protein cloned into an expression vector (pET28b). I would like to express the two domains as separate genes but in the same vector. I though that I could do it by PCR using two primers, one 5'-3' that anneals with the start of the second dom...