Search found 3 matches

by kutya7890
Thu May 12, 2011 1:26 pm
Forum: Molecular Biology
Topic: transgenic mouse
Replies: 5
Views: 3157

Re: transgenic mouse

sorry if it was not clear. the construct will look like this (at least that is what i hope) 5' gene of interest-stop-ires-egfp-cre-ert2-3' utr' so this is one mrna, two proteins, and the second one is a triple fusion protein. the question is: the stop codon is at the and of the cds where should i pl...
by kutya7890
Wed May 11, 2011 2:48 pm
Forum: Molecular Biology
Topic: transgenic mouse
Replies: 5
Views: 3157

Re: transgenic mouse

thanks. yes, it would be a fusion protein. I have my gene of interest, at the end of the CDS there is a stop codon, later i wanna insert an IRES-egfp-cre-ert2 triple fusion protein. I was wondering whether in the 3'UTR insertion my fusion protein disturbes the mRNA of my native protein or it is fine...
by kutya7890
Wed May 11, 2011 9:34 am
Forum: Molecular Biology
Topic: transgenic mouse
Replies: 5
Views: 3157

transgenic mouse

Hi guys, i have a question, might be very stupid. I wanna generate a mouse which is a reporter so after my gene of interest there would be ires gfp. my question is that the CDS contains the stop codon at the end but the mRNA continues. where do i insert my ires gfp. dierctly after the stop codon of ...