Search found 7 matches

by ahkian82
Thu Jan 25, 2007 9:44 am
Forum: Molecular Biology
Topic: Question about DNA conformation
Replies: 4
Views: 3424

By the way, Tris is used as buffer in control the pH in physiological range, near neutral, but not below 7.2 (If i not mistaken)..
by ahkian82
Thu Jan 25, 2007 9:40 am
Forum: Molecular Biology
Topic: Question about DNA conformation
Replies: 4
Views: 3424

Lilkim, Thanks for your suggestion...haha.. It's difficult to do biological-related work in a chemistry lab.. ^_^ . We don't have the room for doing the mixing in such temperature. So, I usually prepare the solution in fresh and use it within one to two hours after preparation.. However, if i just l...
by ahkian82
Mon Jan 22, 2007 6:40 am
Forum: Molecular Biology
Topic: Question about DNA conformation
Replies: 4
Views: 3424

Question about DNA conformation

Does the conformation of both native and denatured DNA show any difference? I'm looking for double-stranded DNA which is in helix form in solution. Recently, i'd bought a calf-thymus DNA from Invitrogen. In the product description, it's said that "...the DNA is prepared from highly pure, phenol...
by ahkian82
Thu Oct 26, 2006 12:29 am
Forum: Molecular Biology
Topic: DNA sedimentation?
Replies: 0
Views: 1082

DNA sedimentation?

I started to work with DNA and a new inorganic complex. The complex and the biopolymer are soluble in the current solvent system (20% DMSO / Tris-HCl 5mM; NaCl 50mM pH 7.2) to give a clear solution. The problem is that when i mixed up my inorganic complexes with the DNA molecules, some precipitate (...
by ahkian82
Mon Sep 18, 2006 5:04 am
Forum: Molecular Biology
Topic: DNA cleavage???
Replies: 0
Views: 994

DNA cleavage???

Dear all, Hi, I have a question which i think i need some advice from you all. Thanks at the very beginning. The question is as below: For the UV-Vis absorption of monomer and double-stranded helical DNA, the latter has a lower absorbance compared to the monnomer. So, if the absorbance of metal comp...
by ahkian82
Tue Aug 08, 2006 3:22 am
Forum: Molecular Biology
Topic: DNA stable in DMSO-H2O system?
Replies: 0
Views: 958

DNA stable in DMSO-H2O system?

In the effort of determining the binding properties of some inorganic complexes with calf thymus DNA, i have come across a solubility problem. Some of the complexes are barely soluble in aqueous solution. I noticed that DMSO can be used to aid dissolution, but how stable is the DNA in this kind of m...
by ahkian82
Wed May 31, 2006 3:10 pm
Forum: Molecular Biology
Topic: DNA stability
Replies: 0
Views: 967

DNA stability

NaCl is used to keep DNA in helix form for the DNA binding studies with inorganic complexes. However, if the use of Cl- source is to be avoided, is there any alternative, for example using sodium acetate, for the same purpose of maintaining the helix structure of the DNA? What are the individual rol...