Search found 3 matches

by shilpagoel
Fri Mar 25, 2011 9:55 pm
Forum: Molecular Biology
Topic: mRNA Search for RT-PCR (U to T)
Replies: 8
Views: 11069

Hi SLKbride ,
Thanks for the reply. If this is a DNA sequence then I would not be able to design primers from it. Correct?
would you be able to pase a link for a mRNA sqequence, any protein will be all right?
by shilpagoel
Fri Mar 25, 2011 3:05 pm
Forum: Molecular Biology
Topic: mRNA Search for RT-PCR (U to T)
Replies: 8
Views: 11069

Sorry for the broken link, The correct link is For eNOS mRNA m For PDGFRbeta mRNA m 1) Can you kindly please post a link to any mRNA sequence from ncbi website form nucleotite ,genbank etc which has U and not T? 2) Can you please confirm that the sequence in the above link can be used for RT PCR pri...
by shilpagoel
Wed Mar 23, 2011 4:35 pm
Forum: Molecular Biology
Topic: mRNA Search for RT-PCR (U to T)
Replies: 8
Views: 11069

mRNA Search for RT-PCR (U to T)

Hi All, This probably will be rated as the most silly question. I am trying to design primers for RT-PCR. I am assuming here that I will have to find an mRNA sequence of interest and then design complementary primers for the mRNA sequence. When I search for mRNA sequence I get sequences that do not ...