Search found 2 matches

by techbrit
Tue Mar 15, 2011 12:24 pm
Forum: Molecular Biology
Topic: Please help - Why can these can/cannot be ligated?
Replies: 1
Views: 2745

Please help - Why can these can/cannot be ligated?

1. Which of the following pairs could be ligated together? (All ter- mini are cohesive and complementary.) a. 5'-dephosphorylated linear insert DNA + linear vector b. Supercoiled vector + linear insert DNA c. 5'-dephosphorylated linear vector + linear 5'-dephosphorylated insert DNA d. Linear 5'-deph...
by techbrit
Sun Mar 13, 2011 7:34 pm
Forum: Molecular Biology
Topic: Help Needed - Ligation Pairing (Vector and DNA)
Replies: 0
Views: 3153

Help Needed - Ligation Pairing (Vector and DNA)

Hello I am a first yr biotech grad student with no biology background. I am struggling understanding the question below Can you please help me understand the reasoning behind these questions and how to understand the concept. Any advice, urls, would be appreciated. This experiment was cloning a kana...