Search found 3 matches

by bluetutu
Mon Mar 14, 2011 2:07 pm
Forum: Molecular Biology
Topic: A problem for miniprep
Replies: 2
Views: 2810

Thanks a lot. I will try XL1 blue, DH5a I tried twice for transformation, but both failed. XL1blue might be a good way...God bless me...


Thanks again^^
by bluetutu
Sun Mar 13, 2011 7:15 pm
Forum: Molecular Biology
Topic: A problem for miniprep
Replies: 2
Views: 2810

A problem for miniprep

Hello Everybody^^, Very nice to find this forum^^. I have a question about my recent work. I did some cloning work these days, and Yesterday, I did miniprep from a BL21 culture, but it seemed contaminated by bacterial genome (complete white on the gel, I changed fresh TEA buffer when doing the Elect...
by bluetutu
Sun Mar 13, 2011 6:58 pm
Forum: Molecular Biology
Topic: Unexpected Fluorescent signal
Replies: 4
Views: 2879

There are two fluorescent phenomena, one is called auto-fluorescent (Phosphorescent), the other is called fluorescent. Auto-fluorescence is a very slow process, it can stay a very long time. Fluorescence is caused by energy emission from fluorophore which is a protein complex like a core surrounded ...