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by joann2717
Thu Mar 10, 2011 3:16 pm
Forum: Molecular Biology
Topic: Reverse transcription (hexamers, decamers or oligo d(T))
Replies: 0
Views: 1129

Reverse transcription (hexamers, decamers or oligo d(T))

Hi I am currently looking into reverse transcription techniques. I am using the MMLV reverse transcriptase kit but wanted to know which primer is best to use. I am unsure of the differences between random hexamers, decamers and oligo d(T) primers. I have isolated mRNA prior to the RT instead of tota...