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- Thu Mar 10, 2011 3:16 pm
- Forum: Molecular Biology
- Topic: Reverse transcription (hexamers, decamers or oligo d(T))
- Replies: 0
- Views: 1129
Hi I am currently looking into reverse transcription techniques. I am using the MMLV reverse transcriptase kit but wanted to know which primer is best to use. I am unsure of the differences between random hexamers, decamers and oligo d(T) primers. I have isolated mRNA prior to the RT instead of tota...