Search found 9 matches

by id10t
Wed Mar 16, 2011 5:40 pm
Forum: Molecular Biology
Topic: physiological assay
Replies: 1
Views: 1356

physiological assay

Does anyone know wat a physiological assay is or how to perform one? Thanks
by id10t
Sat Mar 12, 2011 8:52 pm
Forum: Molecular Biology
Topic: Reporter Genes
Replies: 3
Views: 1766

Re: Reporter Genes

o so a reporter gene is just a tag...it works with any gene?
by id10t
Sat Mar 12, 2011 5:45 am
Forum: Molecular Biology
Topic: Reporter Genes
Replies: 3
Views: 1766

Reporter Genes

How do you know what reporter gene to use when setting up a deletion analysis, or any other experiment?
by id10t
Fri Mar 11, 2011 4:44 am
Forum: Molecular Biology
Topic: esiRNAs repressing retrotransposons
Replies: 0
Views: 1084

esiRNAs repressing retrotransposons

Does anyone know the mechanism in which endogenous siRNA's repress the expression of retrotransposons?
by id10t
Tue Mar 08, 2011 9:37 pm
Forum: Molecular Biology
Topic: transgenes
Replies: 7
Views: 4802

Thanks again that clears some things up...one final question I have is...what are the difference between report genes and transgenes?
by id10t
Tue Mar 08, 2011 5:09 am
Forum: Molecular Biology
Topic: transgenes
Replies: 7
Views: 4802

Thanks for the responses guys...I was also wondering if you could use transgenes for immunoprecipitation experiments? If so how would you?
by id10t
Sun Mar 06, 2011 10:43 pm
Forum: Molecular Biology
Topic: transgenes
Replies: 7
Views: 4802

transgenes

What exactly are transgenes. Do we know the specific sequence of them?
by id10t
Thu Mar 03, 2011 8:04 am
Forum: Molecular Biology
Topic: Determining mRNA sequences
Replies: 2
Views: 3240

Re: Determining mRNA sequences

Well the article is about nonsense mediated decay and how the pre-termination codon recognition is mediated by a competition between 3' UTR associated factors that stimulate or antagonize the recruitment of the Upf complex. It was proposed that mRNA's with long 3' UTRs can evade NMD through the recr...
by id10t
Wed Mar 02, 2011 9:29 pm
Forum: Molecular Biology
Topic: Determining mRNA sequences
Replies: 2
Views: 3240

Determining mRNA sequences

How would you use specific transgenes to determine an mRNA sequence within a 3' UTR? What tests would need to be done?